In vitro evaluation of Sporothrix brasiliensis biotherapic

Sporothrix brasiliensisis one of themost virulent zoonosis which affects animals and humans. This fungus is responsible for subcutaneous infection and its contamination is possible through trauma to the skin. Sporotrichosis is highly prevalent in feline. And Rio de Janeiro appears to have the highest occurrence of cases. Objectives: This study aims to evaluate the in vitroefficacy of Sporothrix brasiliensisbiotherapic, with and without an association to allopathic medicine commonly used in the treatment.Methodology: Conidiumcells of Sporothrix brasiliensiswill be cultured in Potato dextrose agar (PDA) for 5 to 7 days and yeast cells in Brain heart infusion (BHI) for 3 to 5 days. After incubation, the cells will be scraped with a drigalski handle and filtered using cells strainer into a tube and centrifuge for 5 minutes at 3000 RPM. The cells will be resuspended with Phosphate buffered saline (PBS), centrifuge again,and finally resuspended in PBS. After preparing the inocule, the microplates will be prepared. There will be 5groups in vitro. The first one will be the control group, only fungi. The second will be the treatment of fungi with homeopathic medicine (Sporothrix brasiliensis30DH). The third group will be the homeopathic medicine in association with itraconazole. The fourth will be the treatment with itraconazole only. And the last group will be the fungi with dynamized distilled water 30DH. Sporothrix brasiliensis30DHwill be prepared according to Brazilian Homeopathic Pharmacopeia. Results and discussions: The experiments are still in progress and the results will be analyzed through Analysis of variance (ANOVA) to determine statistically significant differences. Previous articles based on biotherapic treatments demonstrated successful results, so our research group is conducting these experiments to evaluate the effect in this model. Conclusion: Experiments will be made to verify the efficacy of biotherapic on sporotrichosis treatments.

Análise histopatológica dos efeitos do bioterápico 200dh em camundongos infectados por Trypanosoma Cruzi / Histopathological analysis of the effects of 200dh biotherapeutic in mice infected with Trypanosoma Cruzi

O Trypanosoma cruzi é um protozoário que instaura uma infecção grave em seu hospedeiro vertebrado, podendo causar danos irreversíveis, principalmente em suas células musculares, conhecida como a doença tripanossomíase ou Doença de Chagas. dicação disponível no mercado, que combate esse parasito, não é completamente eficiente e produz danos indesejáveis e irreversíveis, fazendo-se necessário a busca por outros campos da medicina, em termos de medicação, para combater essa doença. Nesse sentido, pesquisas com medicamentos homeopáticos têm se revelado promissoras no combate ao parasito, sobretudo as formas amastigotas, que são mais difíceis de serem exterminadas. Os bioterápicos são os homeopáticos que obtiveram maior sucesso em relação à infecção por T. cruzi, uma vez que esses medicamentos possuem seu princípio ativo retirado do próprio parasito, suas toxinas, parte de membros, realizando uma terapia conhecida como isoterapia ­ cura pelo igual. Resultados encontrados indicam que o bioterápico 200dH atua coagindo o sistema imune a combater o T. cruzi em cobaias contaminadas, fomentando uma resposta imune celular e humoral mais eficiente do que a fisiológica. Acredita-se que esse medicamento atue estimulando as células de defesa, que passarão a responder de forma antígeno- -específico, favorecendo o combate de amastigotas. Esse processo, provavelmente, é iniciado pela estimulação do macrófago, que por sua vez, de acordo com os resultados encontrados, inicia uma cascata inflamatória, com predominância da via Th1, fomentando a produção de IL4, IL-10 e interferon, auxiliando no combate as amastigotas.

The trypanosoma cruzi is a protozoan that causes a serious infeccion in the vertebrate host, being capable of causing irreversible damages mainly in the muscle cells, also known as trypanosomiasis disease or Chagas Disease. The commercially available drug medication which fights this parasite is not completely efficient and causes undesirable and irreversible damages making it necessary to search for other fields of medicine in terms of medication to combat this disease. Therefore research with homeopathic medicines has being promising in the fight against the parasite, especially the amastigote forms, which are more difficult to be exterminated. Biotherapics are the homeopathic ones that have been more successful in relation to T. cruzi infection, since these drugs have their active principle removed from the parasite itself, its toxins, part of its members, performing a therapy known as isotherapy - cure by the same. The results indicates that the 200dH biotherapic acts by coercing the immune system to fight T. cruzi in contaminated guinea pigs, promoting a cellular and humoral imune response more efficient than the physiological one. It is believed that this medicine works by stimulating the defense cells which will respond in an antigen-specific manner favoring the fight against amastigotes. This process is probably initiated by the stimulation of the macrophage which in turn, according to the remains found, initiates an inflammatory cascade with predominance of the Th1 pathway, promoting the production of IL-4e IL-5 and interferon helping to combat amastigotes

Biotherapic 200dH is harmful to acute murine infection with Trypanosoma cruzi

Biotherapics employed to treat mice infected by Trypanosoma cruzi were carried out with encouraging results. The aim of this study was evaluated the effect of biotherapic of Trypanosoma cruzi 200dH, using two different schedules of treatment. Swiss male mice, aged 56 days-old were infected intraperitonealy with 1,400 blood trypomastigotes of Trypanosoma cruzi Y strain and were divided into groups: C.I.- infected animals, E.D. â€" Infected animals treated from the day 1 until the end of the experiment; 200dH S.D. â€" Infected animals treated on the day 1. Parasitological, clinical and immunological parameters were evaluated. The group of animals that received the medicine in a single dose presented higher value to total parasitaemia and lower value of pre patent period compared to control untreated group (p<0.05), as well as the number of amastigotes which was also higher for this group (S.D.) (p<0.05). Clinically, the S.D.group presented more stable temperature (p<0.05) but not presented another clinical difference among treatments. IL-6 and TNF-α presented similar dosage among treated groups as well as IL-4 and IL-10. IL-17A and INF-γ, presented highest values to S.D. group (p<0.05). All animals died until the 20th day of infection. The lack of improvement in clinical and parasitological parameters, the untimely death and the immunological imbalance display the harmful evolution of the experimental infection by T. cruzi using biotherapic 200dH. The results could be useful for homeopathic physicians. In human clinical use, the choice of dynamizations and treatment schedule should consider acute and chronic diseases to achieve the expected results. (AU)

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Topical administration is a simple and comfortable form of cutaneous administration of drugs. However, in this route of administration the drug needs to overcome the barrier posed by the skin to reach an effective concentration. For this reason, many topical formulations are developed with a cationic component. The promotion of absorption occurs due to the disruption of the stratum corneum. But this cationic component has also high irritating potential to the skin. The biotherapics are medicines prepared from a toxic product or etiologic agent, following the homeopathic pharmacopoeia technique, and they are used mainly in cases of hipersensitization. In this experiment, high dilutions (HD) obtained from a cationic formulation were prepared and evaluated considering cell viability in “in vitro” mouse fibroblast (L929) culture cells model by a colorimetric MTT assay. No signs of toxicity were observed, which demonstrates the safety of these HD preparations to the healthy cells. The effectiveness of these HD was also investigated in cells damaged by cationic formulations. The results demonstrated that the HD 30c was the most effective preparation in preventing the cell damage caused by the tested irritating product, increasing cell viability from 56.6% (damaged cells) to 100% (similar to negative control group, p>0.05). These results provide evidence of the positive action of high dilutions against the exposure to a cytotoxic agent. (AU)

Pilot study: evaluation of homeopathic treatment of Escherichia coli infected swine with identification of virulence factors involved

Aim: To evaluate the efficacy of prophylactic homeopathic treatment of swine diarrhea to achievea satisfactory balance between weight gain and cost. Methods: Escherichia coli (E. coli) wasisolated from 4 piglets with diarrhea, their clinical signs were used to select the adequate homeopathic medicine China officinalis (Chin). Newborn piglets were divided in 4 groups (n=11or 12): control group treated with the routinely used antimicrobial agent (ceftiofur 4 mg 1ml/piglet for 3 days); one group with homeopathic medicine Chin 30 cH; one group treated with biotherapy prepared from locally isolated E. coli; and one group was treated with association ofChin and biotherapy. All isolated samples of E. coli were subjected to polymerase chain reaction(PCR) to identify virulence factors in each group. Results: There was no difference in the numberof animals with diarrhea between groups treated with homeopathic medicine and control; weightgain was greater in groups treated with homeopathic medicine alone and associated withbiotherapy compared to control (p<0.05). Cost of homeopathic treatment was lower compared toceftiofur. Only one E. coli sample used to prepare biotherapy medicine tested positive forvirulence factors (F41); 3 samples from the Chin treated group tested positive for Stbenterotoxins; 1 sample from the homeopathic medicine plus biotherapy treated group and 1 sample from the biotherapy treated group tested positive for F41; 2 samples from the controlgroup tested positive for F41. Conclusion: Strain variability was too large in PCR to allow forany conclusion; despite feces tested positive for E. coli, weight gain of piglets was greater ingroups with homeopathic treatment that thus can be rated more effective in newborn piglets diarrhea.(AU)

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Topical administration is a simple and comfortable form of cutaneous administration of drugs. However, in this route of administration the drug needs to overcome the barrier posed by the skin to reach an effective concentration. For this reason, many topical formulations are developed with a cationic component. The promotion of absorption occurs due to the disruption of the stratum corneum. But this cationic component has also high irritating potential to the skin. The biotherapics are medicines prepared from a toxic product or etiologic agent, following the homeopathic pharmacopoeia technique, and they are used mainly in cases of hipersensitization. In this experiment, high dilutions (HD) obtained from a cationic formulation were prepared and evaluated considering cell viability in “in vitro” mouse fibroblast (L929) culture cells model by a colorimetric MTT assay. No signs of toxicity were observed, which demonstrates the safety of these HD preparations to the healthy cells. The effectiveness of these HD was also investigated in cells damaged by cationic formulations. The results demonstrated that the HD 30c was the most effective preparation in preventing the cell damage caused by the tested irritating product, increasing cell viability from 56.6% (damaged cells) to 100% (similar to negative control group, p>0.05). These results provide evidence of the positive action of high dilutions against the exposure to a cytotoxic agent.

Efeitos de fatores humorais do camundongo tratado com Trypanosominum TC D30, em testes de lise do Tripanosoma cruzi "in vitro" / Humoral factors effects im mice treated with in lysis test of T. cruzi "in vitro"

Utilizando-se soros imune e de animais tratados com Trypanosominum TC D30, em presenca de complemento, observa-se que ocorre a lise de tripomastigotas sanguineos da cepa Y. Quando os referidos soros sao adicionados de soro humano normal inativado, os tripomastigotas sanguineos nao sao lisados. A lise de tripomastigotas mediada por complemento, revela a presenca de anticor-pos liticos no soro imune e no soro de camundongos tratados com o TrypanosominumTC D30, sugerindo a resistencia do hospedeiro contra o T. cruzi

Efeitos de fatores humorais do camundongo tratado com Trypanosominum TC D30, em testes de lise do Tripanosoma cruzi "in vitro" / Humoral factors effects im mice treated with in lysis test of T.cruzi \"in vitro\"

Utilizando-se soros imune e de animais tratados com Trypanosominum TC D30, em presenca de complemento, observa-se que ocorre a lise de tripomastigotas sanguineos da cepa Y. Quando os referidos soros sao adicionados de soro humano normal inativado, os tripomastigotas sanguineos nao sao lisados. A lise de tripomastigotas mediada por complemento, revela a presenca de anticor-pos liticos no soro imune e no soro de camundongos tratados com o TrypanosominumTC D30, sugerindo a resistencia do hospedeiro contra o T. cruzi

Estudo comparativo dos indices de cura de camundongos tratados com quimioterapicos e trypanosominum (TC D 30) / Comparative analysis of the cure rate in mices treated with chemoterapics and trypanosominum (TC D 30)

Trypanosoma cruzi strain isolated from wild mamals or from man and studied in the laboratory show different characteristics that permit their individualization. Blood trypamastigotes exhibit different morphological patterns according to the strain studied. Tropism for different tissues has also been reported. Morphologically, there are wide, thin and intermediate forms wose proportions vary. The results obtained by different investigations in the treatment of Chagas disease are discrepant in different geographic areas, probably because of a relationship with different populations of T.cruzi. In the presentestudy, we investigated experimentally the susceptibility of two polar species, i.e. two species with predominance of thin and wide forms, respectively (Y e Bolivia strains) to the action of Nifurtimax, Benzonidazol and Trypanosaminum (TC D30). Groups of 30 mice aged 60 days were inoculated with 10.4 bloodstream forms of the Y and Bolivia strains of T.cruzi. On the third day of infection, the animals were treated with 100 mg/Kg of both chemotherapeutic agents and with 5 dropsof Trypanosaminum (TC D 30) orally for 20 days. Each animal was evaluated for parasitemia and behavior. Xenodiagnosis and blood cultures were carried out after 40 days. The results showed: a) a sharp resistance of the Bolivia strain to the chemotherapeutic agents, while the Y strain was more susceptible; b) identical susceptibility of the Y and Bolivia strains to treatment with Trypanosominum. We infer from these data that the chemotherapeutic agents act on the parasitic formsby destroying them or not, with sharp differences when different populations areinvolved. In contrast, Trypanosaminum (TC D 30) may act on the stimulating the imunologic response, wich may destroy the etiologic agents through a specific response mechanism, regardless of the characteristics of the strain studied

Ensaios clinicos com o bioterapico "Tripanossoma-cruzi" D30 nas formas cronicas e sintomaticas da "Doenca de Chagas" / Biotherapic trypanosoma cruzi D30 clinical essays in chronical forms of the Disease of Chagas

The auctor presents clinical essays putting out in evidence the action of the biotherapic T.cruzi D30 by chronical forms of the diseaseof Chagas. The initial forms present answers more satisfactoires. Future studieswill must include the utilization of named Similimum

Utilizacao de soros de camundongos e ratos tratados com bioterapico D30 em reacoes de aglutinacao com T. Cruzi / T. Cruzi aglutination reactions utilizing mice serum treated with biotherapic D30

The trypomastigotes were aglutinated by imune animals serum in several dilutions. However, when treated with biotherapic D30, the trypomastigotes were not aglutinate by all dilutions utilized. When diluted 1:2 rat serum aglutination ocorred. The results suggest: the presence of aglutinating antibodies appear soon and is related with the control of the acute phase of humoral immunity

Estudo hispatologico de alteracoes do figado e coracao de camundongos inoculados com T. cruzi e tratados com bioterapico D30 / Histopathologil study in liver and heart of mices infected with T. cruzi and treated with biotherapic D30

The authors studied the action of Biotherapic D30 on liver and heart alterations of mice infected with T.cruzi. Was the liver showed increase cell and nucleus volume, sinusoids with increase size. A diminution of tissue parasitemia were observed on both organs